Anti-prolif. effect of E2 in breast cancer cells that re-exp. ERalpha is mediated by aberr. regulat. of cell cycle genes. Homo sapiens

Gene expression changes caused by estrogen treatment of breast cancer cells that re-express ERalpha was investigated by infecting ER-negative MDA-MB-231 breast cancer cells for 24 h with recombinant adenovirus encoding full-length human ERalpha (Ad-ERalpha) or control vector (Ad-LacZ), and treating them with 0·01% ethanol (vehicle control) or 10-8 M 17beta-estradiol (E2). After 48 h of treatment, total RNA was isolated and used for transcript profiling on Affymetrix GeneChips. Three independent biological replicates of this experiment were carried out. Keywords: ordered

本研究针对重新表达雌激素受体α（ERalpha）的乳腺癌细胞经雌激素处理后诱发的基因表达变化展开探究。实验流程如下：将雌激素受体阴性的MDA-MB-231乳腺癌细胞分别以编码全长人ERalpha的重组腺病毒（Ad-ERalpha）或对照载体（Ad-LacZ）感染24小时，随后分别用0.01%乙醇（溶剂对照）或10^-8 M 17β-雌二醇（E2）进行处理。处理48小时后，提取总RNA并在Affymetrix基因芯片（Affymetrix GeneChips）上开展转录组谱分析（transcript profiling）。本实验共完成3次独立生物学重复。关键词：有序