microRNAs profiling in hippocampal cells of mouse with Pilocarpine-induced Status Epilepticus (SE)

SE was induced in mice (NMRI) chemically by a single dose of Pilocarpine (300mg/kg;i.p.). RNA was extracted from the hippocampi of treated and wild-type mice at 24 h and 28 d after induction. microRNA expressions were measured via microarray technology using Exiqon's miCURY™ LNA Arrays. 4 conditions, Pilocarpine treated mice at 24 h and 28 d, and the control wild-type at the same types, each of 8 replicates.

本研究通过单次腹腔注射（intraperitoneal, i.p.）300mg/kg毛果芸香碱（Pilocarpine），在NMRI品系小鼠中化学诱导癫痫持续状态（Status Epilepticus, SE）模型。分别于造模后24小时与28天，从造模组与野生型对照组小鼠的海马体中提取总RNA。采用Exiqon公司的miCURY™锁核酸（Locked Nucleic Acid, LNA）芯片阵列，通过微阵列技术检测各组小鼠的微小RNA（microRNA）表达水平。本数据集包含4组实验条件：造模后24小时的毛果芸香碱处理组、造模后28天的毛果芸香碱处理组，以及对应时间点的野生型对照组，每组均设置8个生物学重复。