Proteome database of Proteus vulgaris ATCC 49132

Known for its swarming motility, <i>Proteus vulgaris</i> is a Gram-negative, rod-shaped bacterium that inhabits a variety of environments ranging from the human gastrointestinal tract to soil and water. Ability to thrive in a variety of environmental and nutritional environments may partially explain why <i>P. vulgaris </i>is also an opportunistic human pathogen causing, for example, wound infections. But, the defining feature of this bacterium remains its coordinate movement across surfaces known as swarming motility, which endows it with model organism status in this field of research. This work sought to provide some fundamental information of <i>P. vulgaris</i> ATCC 49132 through parsing the organism’s UniProt proteome file through an in-house MATLAB software. Altogether, a proteome database comprising protein name, amino acid sequence, number of residues, molecular weight and nucleotide sequence was constructed that, in addition to providing key features of each protein in <i>P. vulgaris </i>ATCC 49132, also informs researchers of the metabolic and signalling pathways present in the species. Such information should find use in a variety of basic microbiological investigations seeking to decipher the question: why and how <i>P. vulgaris</i> moves on surfaces in coordinated fashion? <br><br>

以群集运动（swarming motility）能力为标志性特征的普通变形杆菌（*Proteus vulgaris*）是一类革兰氏阴性杆状细菌，其生存环境多样，涵盖人类胃肠道、土壤与水体等。该菌能够在多种环境与营养条件下存活增殖，这或许可以部分解释为何普通变形杆菌同时也是一种机会致病性人类致病菌，可引发伤口感染等病症。但该菌最核心的特征仍是其在固体表面的协同运动——即群集运动（swarming motility），这也使其成为该研究领域的经典模式生物。

本研究通过自研MATLAB软件解析普通变形杆菌ATCC 49132的UniProt蛋白质组文件，旨在为该菌株提供基础研究数据。最终构建了包含蛋白质名称、氨基酸序列、残基数量、分子量及核苷酸序列的蛋白质组数据库；该数据库不仅完整呈现了普通变形杆菌ATCC 49132中各蛋白质的关键特性，还揭示了该物种所具备的代谢与信号通路信息。此类研究数据可应用于多项基础微生物学研究，以解答核心科学问题：普通变形杆菌为何能够以协同方式在固体表面运动？其具体的运动机制又是什么？