The roles of CSE1L in epigenetic silencing

Epigenetic silencing can be mediated by various mechanisms and many regulators remain to be identified. We identified CSE1L as a factor essential for silencing of the reporter gene and a fraction of endogenous methylated genes. CSE1L depletion did not cause DNA demethylation or increase of global histone acetylation. Nevertheless, these methylated genes derepressed by CSE1L depletion largely overlapped with methylated genes that were also reactivated by treatment of histone deacetylase inhibitors (HDACi). Overall design: We measured DNA methylation by MeDIP and expression profiles by RNA-seq in B2-1 HEK293 cells and serveral Knockdown/treatment cells.表观遗传沉默（Epigenetic silencing）可通过多种机制介导，目前仍有诸多调控因子有待鉴定。本研究鉴定出CSE1L是介导报告基因及部分内源性甲基化基因沉默的必需因子。CSE1L敲低不会引发DNA去甲基化，也不会导致整体组蛋白乙酰化水平升高。尽管如此，经CSE1L敲低解除抑制的甲基化基因，与经组蛋白去乙酰化酶抑制剂（HDACi）处理后重新激活的甲基化基因存在大量重叠。实验设计：本研究在B2-1 HEK293细胞及数株敲低/处理组细胞中，通过MeDIP检测DNA甲基化水平，并通过RNA-seq分析基因表达谱。