Molecular subtypes in pancreatic adenocarcinoma [tumors, normal and pancreatitis]

The goal of the study was to examine the transcriptional profile of pancreatic tumors to identify molecular subtypes in order to develop validated clinically useful gene expression signature with the potential to guide therapy decision. Tissue was obtained by snap freezing in liquid nitrogen as soon as removed. All tissue samples were stored at -80C. Samples were embedded in OCT and a 4ug section was taken for H/E staining. After QC procedure to ensure high quality RNA (RIN>7) and confirm PDAC histology, 78 samples were subjected to microarray analysis. All patients signed an Institutional Review Board approved consent for bio-banking, clinical data extraction and molecular analysis. 85 samples (77 tumors, 3 normal and 5 pancreatitis) were compared to a mixed reference pool of 66 tumor samples to identify gene expression patterns.

本研究旨在解析胰腺肿瘤的转录组谱（transcriptional profile），以鉴定其分子亚型，进而开发经过验证、可指导临床治疗决策的实用基因表达特征。组织样本在切除后即刻经液氮快速冷冻处理，所有样本均保存于-80℃环境中。样本经OCT包埋后，切取4μm切片进行苏木精-伊红（H/E）染色。在完成质量控制（QC）以确保RNA质量达标（RNA完整性指数RIN>7）并确认病理为胰腺导管腺癌（PDAC）后，共78份样本用于基因芯片（microarray）分析。所有患者均签署了经机构审查委员会（IRB）批准的知情同意书，同意开展生物样本库建库、临床数据提取及分子分析相关研究。本研究将85份样本（含77份肿瘤样本、3份正常样本及5份胰腺炎样本）与由66份肿瘤样本组成的混合参考池进行比对，以识别基因表达模式。