Sequencing SARS-CoV-2 from Antigen Cards

This project demonstrates that it is possible to produce SARS-CoV-2 whole genome sequences from antigen test-derived anterior nares swabs. Viral RNA was extracted from BinaxNOW COVID-19 Ag Card (Abbott Diagnostics Scarborough) swabs and SARS-CoV-2 whole genome sequence libraries were prepared using an amplicon-based NGS panel (Paragon Genomics: CleanPlex SARS-CoV-2 FLEX Panel). Prepared libraries were then sequenced on the MiSeq system at 2 x 151 bp using the MiSeq v3, (600 cycle) kit (Illumina, Cat. MS-102-3003).

本研究证实，可从经抗原检测的前鼻拭子中获取新型冠状病毒（SARS-CoV-2）的全基因组序列。研究人员从BinaxNOW新冠抗原检测卡（Abbott Diagnostics Scarborough）配套拭子中提取病毒RNA，并采用基于扩增子的下一代测序（Next Generation Sequencing，NGS）试剂盒（Paragon Genomics：CleanPlex SARS-CoV-2 FLEX Panel）构建SARS-CoV-2全基因组测序文库。制备完成的测序文库随后在MiSeq测序平台上，使用MiSeq V3（600循环）测序试剂盒（Illumina，货号MS-102-3003）以2×151 bp的双端测序模式完成测序。