Gene expression profile at single cell level of plasma cells (PCs) from human bone marrow. Gene expression profile at single cell level of plasma cells (PCs) from human bone marrow

Antibody-secreting plasma cells (PCs) are generated in secondary lymphoid organs, but are reported to reside in an emerging range of anatomical sites. Analysis of the transcriptome of different tissue-resident (Tr)PC populations revealed that they each have their own transcriptional signature indicative of functional adaptation to the host tissue environment. In contrast to expectation, all TrPCs were extremely long-lived, regardless of their organ of residence, with the longevity influenced by intrinsic factors like the immunoglobulin isotype. Analysis at single-cell resolution revealed that the bone marrow is unique in housing a compendium of PCs generated all over the body that retain aspects of the transcriptional program indicative of their tissue of origin. Overall design: Plasma cells were isolated from the bone marrow of a healthy adult human donor and analyzed using scRNAseq.

抗体分泌浆细胞（antibody-secreting plasma cells, PCs）可在次级淋巴器官中生成，但现有研究表明它们可定植于多种新兴的解剖部位。对不同组织驻留浆细胞（tissue-resident plasma cells, TrPCs）群体的转录组分析显示，各类群体均拥有独特的转录特征，提示其可通过功能适配以适应宿主组织的微环境。与此前预期相悖的是，所有组织驻留浆细胞均具有极长的存活周期，且与其定植的器官无关，其存活时长受免疫球蛋白同种型等内在因素调控。单细胞分辨率分析显示，骨髓具有独特性：它可容纳全身各处生成的浆细胞集群，且这些浆细胞仍保留与其起源组织相关的部分转录程序特征。
实验整体设计：从一名健康成年人类供者的骨髓中分离浆细胞，并通过单细胞RNA测序（single-cell RNA sequencing, scRNAseq）完成分析。