Pax6 mutant neocortex vs. wild type. Mus musculus

Pax6 is a transcription factor with key functional roles in embryonic development. In order to identify downstream effectors of Pax6 in the developing cerebral cortex we performed microarray analysis. We compared gene expression profiles of cortical tissues isolated from wild type and Pax6-/- mouse embryos. Overall design: In order to identify Pax6 downstream targets we carried out microarray analysis of Pax6-/- mutant mice. Pax6 is highly expressed in the mouse cerebral cortex at embryonic day E14.5, therefore we selected this tissue in order to compare gene expression profiles between wild type and Pax6-/- homozygous cortici. RNA samples were isolated from three mutant and three wild type embryos.

Pax6是一种转录因子，在胚胎发育过程中发挥关键功能作用。为鉴定发育中大脑皮层内Pax6的下游效应因子，我们开展了微阵列（microarray）分析。我们对比了从野生型（wild type）与Pax6-/-小鼠胚胎中分离的皮层组织的基因表达谱。实验总体设计：为鉴定Pax6的下游靶标，我们对Pax6-/-突变小鼠开展了微阵列分析。Pax6在小鼠胚胎发育第14.5天（E14.5）的大脑皮层中呈高表达状态，因此我们选取该组织，用于对比野生型与Pax6-/-纯合突变皮层的基因表达谱。我们从3只突变型胚胎与3只野生型胚胎中分离得到了RNA样本。