Circadian parameters obtained from EIF2S1 temperature compensation RNAi screen.

Experiments have been performed as described in Materials and methods. Raw bioluminescence recordings are detrended by a 24-hour moving average and analyzed via the ChronoStar software. Results of the nonlinear fitting by ChronoStar are depicted in the last 6 columns of each data block. Data where the bioluminescence after transduction and selection of U-2 OS cells is low compared to average (red font) as well as those where the error of the nonlinear curve fit is larger than 0.3 (red shaded cells) are sorted out for the further analysis in S11 Fig. Note that sheet 1 contains results for shRNA constructs targeting EIF2S1, while sheet 2 contains results for the nonsilencing constructs. (XLSX)