KRAS and MEK activation promote antisense oligonucleotide uptake and activity by limiting collagen production

Using RNAseq we describe transcriptional changes downstream of MEK1 affecting RNA kncokdown following free uptake RNaseH1 gapmer antisense oligonucleotides Overall design: We perform RNAseq on cell lines with a stimulated MAPK pathway via inducible expression of KRAS(G12D), inducible expression of MEK1(S218/S222DD) and by stimulation with the PKC activator, PMA

本研究通过RNA测序（RNAseq）解析了丝裂原活化蛋白激酶激酶1（MEK1）下游的转录组变化，该变化可影响自由摄取型RNaseH1 gapmer反义寡核苷酸介导的RNA敲降效应。整体实验设计：我们对三类丝裂原活化蛋白激酶（MAPK）通路激活的细胞系进行RNA测序，分别为通过诱导型表达KRAS(G12D)激活通路的细胞系、通过诱导型表达MEK1(S218/S222DD)激活通路的细胞系，以及经蛋白激酶C（PKC）激活剂PMA（佛波醇12-十四酸酯13-乙酸酯）刺激的细胞系。