Data from: De novo transcriptome assemblies of four accessions of the metal hyperaccumulator plant Noccaea caerulescens

Noccaea caerulescens of the Brassicaceae family has become the key model plant among the metal hyperaccumulator plants. Populations/accessions of N. caerulescens from geographic locations with different soil metal concentrations differ in their ability to hyperaccumulate and hypertolerate metals. Comparison of transcriptomes in several accessions provides candidates for detailed exploration of the mechanisms of metal accumulation and tolerance and local adaptation. This can have implications in the development of plants for phytoremediation and improved mineral nutrition. Transcriptomes from root and shoot tissues of four N. caerulescens accessions with contrasting Zn, Cd and Ni hyperaccumulation and tolerance traits were sequenced with Illumina Hiseq2000. Transcriptomes were assembled using the Trinity de novo assembler and were annotated and the protein sequences predicted. The comparison against the BUSCO plant early release dataset indicated high-quality assemblies.The predicted protein sequences have been clustered into ortholog groups with closely related species. The data serve as important reference sequences in whole transcriptome studies, in analyses of genetic differences between the accessions and other species, and for primer design.

十字花科（Brassicaceae）的天蓝遏蓝菜（Noccaea caerulescens）已成为金属超富集植物领域的核心模式植物。来自土壤金属浓度存在差异的不同地理区域的天蓝遏蓝菜居群/种质系，在金属超富集与超耐受能力方面表现出显著差异。对多个居群的转录组（transcriptome）进行比较分析，可为深入解析金属积累、耐受机制以及本地适应过程提供候选研究靶点。该研究成果可为植物修复专用作物的培育以及矿物营养性状改良提供理论支撑。本研究针对4个锌（Zn）、镉（Cd）与镍（Ni）超富集及耐受性状差异显著的天蓝遏蓝菜居群的根和茎组织转录组，采用Illumina Hiseq2000平台完成测序。转录组组装使用Trinity从头组装软件（Trinity de novo assembler）完成，并进行了功能注释与蛋白质序列预测。与BUSCO植物早期发布数据集的比对结果表明，本次组装的转录组质量优异。预测获得的蛋白质序列已与近缘物种的序列聚类为直系同源基因簇（ortholog groups）。该数据集可作为全转录组研究、居群间及跨物种遗传差异分析以及引物设计的重要参考序列。