Protopterus dolloi DICE microCT data for Mandibular musculature constrains brain-endocast disparity in sarcopterygians

The specimen was stained in IKI using the method of Metscher (2009) for three months.<br> p { margin-bottom: 0.25cm; direction: ltr; color: #000000; line-height: 115%; text-align: left; orphans: 2; widows: 2; background: transparent } p.western { font-family: "Calibri", serif; font-size: 11pt; so-language: en-AU } p.cjk { font-family: "Calibri"; font-size: 11pt; so-language: en-US } p.ctl { font-family: "DejaVu Sans"; font-size: 11pt; so-language: ar-SA } a:link { color: #0000ff; text-decoration: underline } Micro-CT (uCT) scans were obtained using a Feinfocus 10-160kV transmission X-ray source and a Perkin Elmer XRD0822 1 MP flat panel X-ray camera. The peak energy used was 120kV, and 2 second exposures were corrected with offset and gain images. The resulting exposures were reconstructed as a tif stack using OctopusV9 resulting in a voxel size of 86.9 microns. p { margin-bottom: 0.25cm; direction: ltr; color: #000000; line-height: 115%; text-align: left; orphans: 2; widows: 2; background: transparent } p.western { font-family: "Calibri", serif; font-size: 11pt; so-language: en-AU } p.cjk { font-family: "Calibri"; font-size: 11pt; so-language: en-US } p.ctl { font-family: "DejaVu Sans"; font-size: 11pt; so-language: ar-SA } a:link { color: #0000ff; text-decoration: underline }

样本采用Metscher（2009）的方法，使用碘-碘化钾（IKI）染色剂染色三个月。显微计算机断层扫描（Micro-CT，简称uCT）数据采集使用Feinfocus 10-160kV透射X射线源与Perkin Elmer XRD0822型1百万像素平板X射线相机，扫描峰值电压设为120kV，2秒曝光图像通过偏移校正图像与增益校正图像完成校正。采集得到的曝光数据通过OctopusV9软件重建为tif stack格式图像序列，最终体素尺寸为86.9微米。