Global gene expression analysis of three different types of mouse motor neurons

Analysis of gene expression differences in three types of mouse motor neurons: (1) those harvested from E13.5 mouse embryos; (2) those derived from embryonic stem cells by directed differentiation; and (3) those made from mouse embryonic fibroblasts by transcription factor reprogramming. Hb9::GFP+ mouse motor neurons were obtained by three methods [(1) dissection of Hb9::GFP-transgenic E13.5 mouse embryo spinal cord; (2) directed differentiation of an Hb9::GFP-transgenic mouse embryonic stem cell line, V6.5; (3) reprogramming of Hb9::GFP-transgenic mouse embryonic fibroblasts by overexpression of 10 transcription factors]. Each type of motor neuron was purified by FACS and harvested in Trizol. Total RNA was purified and prepared for hybridization onto Illumina MouseRef-8.

三种小鼠运动神经元的基因表达差异分析：(1) 从E13.5小鼠胚胎中采集的运动神经元；(2) 经定向分化由胚胎干细胞诱导获得的运动神经元；(3) 通过转录因子重编程由小鼠胚胎成纤维细胞制备的运动神经元。本研究通过三种方法获取Hb9::GFP阳性（Hb9::GFP+）小鼠运动神经元：(1) 解剖Hb9::GFP转基因E13.5小鼠胚胎的脊髓；(2) 定向分化Hb9::GFP转基因小鼠胚胎干细胞系V6.5；(3) 通过过表达10种转录因子，对Hb9::GFP转基因小鼠胚胎成纤维细胞进行重编程。各类型运动神经元均通过荧光激活细胞分选（FACS）完成纯化，随后以Trizol试剂收集细胞。提取总RNA并制备杂交样本，用于在Illumina MouseRef-8芯片上进行杂交。