Data of "High-speed cryo-microscopy proves that ice-nucleating proteins of Pseudomonas syringae trigger freezing at hydrophobic interfaces"

Raw data of a study titled "High-speed cryo-microscopy proves that ice-nucleating proteins of Pseudomonas syringae trigger freezing at hydrophobic interfaces". The onset_locations.zip folder contains all analyzed images which are screenshots from the cryo-microscopic videos. Raw screenshots and evaluated images are included in two sub-folders per samples. The sample description is the name of the folders. The ice_propogation_velocity.zip folder contains all images that were used for the evaluation of the propagation velocity of ice. Every sample folder contains the original spot detection image, one image at a later time point, the subtracted image, and one image with the measured distance of the ice front indicated as white scale bar. The Results_(ice_propagation_velocity).xlsx contains the results from the velocity calculations, the Results_(surface tension).xlsx contains the evaluated surface tension values and the Results_(temperatures and locations).xlsx file contains all evaluated freezing locations (polar coordinates) and temperatures of all analyzed samples.

本数据集为一项题为《高速冷冻显微镜证实丁香假单胞菌（Pseudomonas syringae）的冰核蛋白（ice-nucleating proteins）可在疏水界面（hydrophobic interfaces）触发结冰》的研究的原始数据。其中，onset_locations.zip压缩包内含所有经分析的图像，这些图像均为冷冻显微镜视频的截图。每个样本对应两个子文件夹，分别存放原始截图与经评估处理后的图像，样本名称即为对应文件夹的名称。ice_propogation_velocity.zip压缩包内含所有用于评估冰传播速度的图像。每个样本文件夹中均包含原始斑点检测图像、一张后续时间点的图像、差值图像，以及一张标注有以白色比例尺形式呈现的冰锋（ice front）实测距离的图像。《Results_(ice_propagation_velocity).xlsx》包含冰传播速度计算所得的结果；《Results_(surface tension).xlsx》收录经评估得到的表面张力数值；《Results_(temperatures and locations).xlsx》则存储了所有经分析样本的结冰位点（极坐标）与温度的全部评估数据。