Elephas maximus (Asian elephant) genome, mEleMax1, primary haplotype. Elephas maximus isolate:mEleMax1

The Indian elephant is one of the three subspecies of the Asian Elephant. It has been classified as Endangered on the IUCN realist since 1986 due to habitat loss and environmental degradation mainly caused by human activities. This is the primary assembly of the mEleMax1. This assembly has been produced as part of the VGP Project, and supported by Virpi Lummaa (Professor, Ecology and Evolutionary Biology, university of Turku, Finland), Camila Mazzoni (group leader at the Leibniz Institute for Zoo and Wildlife Research, Berlin, Germany), and by Colossal Biosciences. Work was done in collaboration with Sara Ord, Eriona Hysolli, and Larissa Souza Arantes. Skin tissue was collected from a 50 years old adult male at the San Diego zoo, California (SB-218, and GAN # 26734787; ZIMS/Species 360) and fibroblast cells were cultured by the San Diego Frozen Zoo. The sample was sequenced using the VGP 2.0 individual pipeline at the Rockefeller University Vertebrate Genome Lab, led by Olivier Fedrigo and Erich Jarvis. The primary haplotype contigs were generated from PacBio Hifi reads, scaffolded with Bionano optical maps, and Arima HiC reads. The genome was assembled by Diego De Panis. Manual curation of chromosomes and other genomic features was conducted by Nadolina Brajuka and led by Giulio Formenti.

印度象（Indian elephant）是亚洲象（Asian Elephant）的三个亚种之一。自1986年起，因其主要受人类活动引发的栖息地丧失与环境退化影响，该物种已被列入国际自然保护联盟（IUCN）红色名录（IUCN Red List）的濒危（Endangered）等级。本数据集为mEleMax1的核心基因组组装结果。该组装成果为脊椎动物基因组计划（Vertebrate Genomes Project, VGP）的一部分，得到了芬兰图尔库大学生态与进化生物学教授Virpi Lummaa、德国柏林莱布尼茨动物园与野生动物研究所团队负责人Camila Mazzoni，以及Colossal Biosciences公司的支持。本工作由Sara Ord、Eriona Hysolli与Larissa Souza Arantes协作完成。样本采集自美国加利福尼亚州圣迭戈动物园一只50岁成年雄性个体的皮肤组织（样本编号：SB-218，GAN编号：26734787；ZIMS/Species 360），成纤维细胞由圣迭戈冷冻动物园（San Diego Frozen Zoo）完成培养。该样本由美国洛克菲勒大学脊椎动物基因组实验室（由Olivier Fedrigo与Erich Jarvis牵头）采用VGP 2.0个体测序流程完成测序。核心单倍型重叠群通过PacBio HiFi测序读长生成，并借助Bionano光学图谱与Arima Hi-C测序读长完成支架组装。该基因组的组装工作由Diego De Panis完成。染色体与其他基因组特征的人工注释工作由Nadolina Brajuka开展，并由Giulio Formenti牵头。