SAMMY-seq, H3K9me3 and H3K27me3 ChIP-seq and RNA-seq of control and progeria fibroblasts

We present a new high-throughput sequencing-based technique, named Sequential Analysis of MacroMolecules accessibilitY (SAMMY-seq), for the genome-wide mapping of chromatin regions separated by differential accessibility. The method is based on the sequential extraction of multiple chromatin fractions, corresponding to increasingly compacted and less accessible chromatin regions, which are mapped along the genome using high-throughput sequencing. Using SAMMY-seq we analyzed Hutchinson-Gilford progeria syndrome (HGPS) skin fibroblasts and normal control fibroblasts. Additionally we carried out ChIP-seq for the H3K9me3 and H3K27me3 histone modifications and RNA-seq for the characterization of transcriptome changes on the same HGPS and control fibroblasts. Using SAMMY-seq we analyzed Hutchinson-Gilford progeria syndrome (HGPS) skin fibroblasts and normal control fibroblasts. Additionally we carried out ChIP-seq for the H3K9me3 and H3K27me3 histone modifications and RNA-seq for the characterization of transcriptome changes on the same HGPS and control fibroblasts. Human raw data will be uploaded to dbGaP

本研究提出一种基于高通量测序的全新技术，命名为大分子可及性序贯分析（Sequential Analysis of MacroMolecules accessibilitY，SAMMY-seq），用于全基因组范围内绘制可及性存在差异的染色质区域。该技术通过序贯提取多组染色质组分得以实现，这些组分对应着逐渐致密、可及性逐步降低的染色质区域，随后通过高通量测序完成其全基因组定位。本研究利用SAMMY-seq技术对哈钦森-吉尔福德早衰综合征（Hutchinson-Gilford progeria syndrome，HGPS）皮肤成纤维细胞与正常对照成纤维细胞进行了分析；此外，针对同一批HGPS细胞与对照细胞，我们开展了针对H3K9me3、H3K27me3组蛋白修饰的ChIP-seq实验，以及用于表征转录组变化的RNA-seq实验。本研究再次利用SAMMY-seq技术对哈钦森-吉尔福德早衰综合征（HGPS）皮肤成纤维细胞与正常对照成纤维细胞进行了分析；此外，针对同一批HGPS细胞与对照细胞，我们开展了针对H3K9me3、H3K27me3组蛋白修饰的ChIP-seq实验，以及用于表征转录组变化的RNA-seq实验。人类原始数据将上传至dbGaP数据库。