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Relevant studies indicate that coagulation-related genes (CGs) are associated with the development and progression of ischemic stroke (IS). This study aimed to detect differentially expressed coagulation genes in IS patients using gene sequencing technology and perform bioinformatics analysis. A comparison between the IS group and the control group revealed a total of 1,045 differentially expressed genes (DEGs), including 847 upregulated genes and 198 downregulated genes. Ultimately, four coagulation-related genes were determined. Results from the disease risk prediction model demonstrated that APBB1IP, CR1, F5, and VASP possess diagnostic value for IS. Focusing on these four key genes, we constructed a competing endogenous RNA (ceRNA) network. Furthermore, immune infiltration analysis revealed that decreases in naive CD4+ T cells and M2 macrophages, along with increases in activated memory CD4+ T cells and neutrophils, are likely involved in the pathogenesis of IS.

已有相关研究表明，凝血相关基因（coagulation-related genes, CGs）与缺血性脑卒中（ischemic stroke, IS）的发生发展密切相关。本研究旨在通过基因测序技术检测缺血性脑卒中患者体内的差异表达凝血基因，并开展生物信息学分析。将缺血性脑卒中组与对照组进行比对，共筛选得到1045个差异表达基因（differentially expressed genes, DEGs），其中上调基因847个，下调基因198个。最终确定4个凝血相关基因。疾病风险预测模型的结果显示，APBB1IP、CR1、F5及VASP对缺血性脑卒中具有诊断价值。针对这4个关键基因，我们构建了内源竞争RNA（competing endogenous RNA, ceRNA）调控网络。此外，免疫浸润分析结果表明，初始CD4+T细胞与M2型巨噬细胞的浸润减少，以及活化记忆性CD4+T细胞和中性粒细胞的浸润增加，可能参与了缺血性脑卒中的发病机制。