CIL:10008, Didinium nasutum, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: film; Print from negative scanned for Photoshop.

样品制备：戊二醛固定组织（glutaraldehyde fixed tissue）、四氧化锇固定组织（osmium tetroxide fixed tissue）、环氧树脂包埋组织（epoxy resin embedment tissue）、切片机切片组织（microtome-sectioned tissue）
与完整细胞的对应关系：切片机切片组织（microtome-sectioned tissue）
成像类型：透射电子显微镜（transmission electron microscopy, TEM）；电子束照明
成像模式：电子检测（detection of electrons）；胶片成像
成像参数：电子密度（electron density）
衬度来源：广谱特异性染色（stain with broad specificity）
可视化方法：广谱特异性染色、四氧化锇、铀盐（uranyl salt）、铅盐（lead salt）
图像处理流程：胶片成像；负片扫描后经Photoshop软件处理并打印。