Pseudomonas simiae WCS417 gene expression profiles in response to root exudates from Arabidopsis wild-type and defense barrier mutants

The beneficial interactions between Arabidopsis and Pseudomonas simiae WCS417 changed when several root defense barriers cannot function properly, including cutin, suberin, lignin, callose, camalexin and glucosinolates. To test the effects of root exudates of these defense barrier mutants on the function of WCS417, we exposed WCS417 to root exudates of wild-type or each of the mutants for 1 h, after which we profiled bacterial transcriptome by RNA sequencing.

拟南芥（Arabidopsis）与类人假单胞菌（Pseudomonas simiae）WCS417之间的有益互作，在多种根系防御屏障无法正常发挥功能时会发生改变，这些防御屏障包括角质（cutin）、木栓质（suberin）、木质素（lignin）、胼胝质（callose）、植保素（camalexin）与硫代葡萄糖苷（glucosinolates）。为探究上述防御屏障突变体的根系分泌物对WCS417菌株功能的影响，我们将WCS417分别暴露于野生型（wild-type）或各突变体的根系分泌物中处理1小时，随后通过RNA测序（RNA sequencing）分析细菌转录组。