RNA-Sequencing for Additional Molar Germs Transcriptomes at Stages of Early Development in Miniature Pigs. Sus scrofa

The molecular mechanisms of the regulatory network of mRNAs and long non-coding RNAs during sequential formation of additional molars remain poorly characterized in diphyodont mammals. The diphyodont miniature pig has proved to be a valuable model for studying human molar morphogenesis. Here, we performed RNA-seq on miniature pigs at three molar developmental stages to examine their differential gene expression profiles and potential regulatory networks during additional molar morphogenesis. Many new unannotated genes plus putative long non-coding RNAs were identified. Our data provide fundamental knowledge and a basis for understanding the molecular mechanisms governing cascade initiation of additional molars. Overall design: Additional molar germs mRNA profiles from early developmental stages at E50, E60 and E70 in Miniature pigs' were generated by deep sequencing using Illumina Hiseq 2000

双型齿哺乳动物（diphyodont mammals）中，额外磨牙依次形成过程内，信使RNA（messenger RNA，mRNA）与长链非编码RNA（long non-coding RNAs）的调控网络分子机制目前仍未得到充分阐明。双型齿小型猪已被证实为研究人类磨牙形态发生（molar morphogenesis）的理想动物模型。本研究对处于三个磨牙发育阶段的小型猪开展RNA测序（RNA-seq），以分析额外磨牙形态发生过程中的差异基因表达谱及潜在调控网络。本研究共鉴定出大量新的未注释基因及推定长链非编码RNA，相关数据为阐明调控额外磨牙级联启动的分子机制提供了基础认知与研究依据。整体实验设计：本研究通过Illumina HiSeq 2000测序平台进行深度测序，获取了小型猪胚胎第50天（E50）、第60天（E60）及第70天（E70）三个早期发育阶段的额外磨牙牙胚的mRNA表达谱。