In situ promoter capture Hi-C data of differentiated SGBS human preadipocytes (days 0, 2, 8 and 16 of differentiation to adipocytes) and iPSC-derived hypothalamic neurons (days 12, 16 and 27 of differentiation). In situ promoter capture Hi-C data of differentiated SGBS human preadipocytes (days 0, 2, 8 and 16 of differentiation to adipocytes) and iPSC-derived hypothalamic neurons (days 12, 16 and 27 of differentiation)

Human SGBS preadipocytes were differentiated into adipocytes, and human iPSCs were differentiated into hypothalamic neurons. Cells were collected for in situ promoter capture Hi-C [PMID: 29988018] at several differentiation stages. The differentiations were performed in one biological replicate, with two technical replicates (different wells of the differentiation that were also processed individually during library preparation). SGBS Day0: Represents the preadipocyte state. SGBS Day2: Represents immature adipocytes. SGBS Day8: Represents early mature adipocytes. SGBS Day16: Represents mature adipocytes. Hypothalamic Day 12: Represents early hypothalamic neurons. Hypothalamic Day 16: Represents mid hypothalamic neurons. Hypothalamic Day 27: Represents mature hypothalamic neurons.

本研究将人SGBS脂肪前体细胞诱导分化为脂肪细胞，并将人诱导多能干细胞（induced pluripotent stem cells, iPSCs）诱导分化为下丘脑神经元。在多个分化阶段收集细胞，用于开展原位启动子捕获Hi-C（promoter capture Hi-C）实验，相关方法参考文献[PMID: 29988018]。所有分化实验均设置1次生物学重复，并配套2次技术重复（即分化体系的不同孔板，在文库制备阶段单独进行处理）。具体各时间节点对应的细胞状态如下：
SGBS第0天：代表脂肪前体细胞状态；
SGBS第2天：代表未成熟脂肪细胞；
SGBS第8天：代表早期成熟脂肪细胞；
SGBS第16天：代表成熟脂肪细胞；
下丘脑神经元第12天：代表早期下丘脑神经元；
下丘脑神经元第16天：代表中期下丘脑神经元；
下丘脑神经元第27天：代表成熟下丘脑神经元。