In vitro modeling of primordial germ cell development. Mus musculus

Transgenic StellaGFP ESCs were used to derive primordial germ cells during embryoid body (EB) differentiation, and microarry analysis used to compared FACS sorted Stella-positive cells of day 7 Ebs with the parental ESCs and Stella-negative cells of day 7 Ebs. Keywords: in vitro, primordial germ cells, embryonic stem cells, stella Overall design: Four replicates of Stella-positive day 7 cells were compared to two replicates of stella-negative day 7 cells. Also, there were three replicated of FACS sorted Stella-negative ESCs, two replicated of FACS-sorted Stella-postive ESCs, and two replicates of unsorted StellaGFP ESCs.

本研究利用转Stella基因绿色荧光蛋白标记的胚胎干细胞（Embryonic Stem Cells, ESCs），在类胚体（embryoid body, EB）分化过程中诱导产生原始生殖细胞（primordial germ cells）；采用微阵列（microarray）分析，对第7天类胚体中经荧光激活细胞分选（Fluorescence-Activated Cell Sorting, FACS）获得的Stella阳性细胞，与其亲本胚胎干细胞以及第7天类胚体中的Stella阴性细胞进行了比较。
关键词：体外（in vitro）、原始生殖细胞、胚胎干细胞、Stella
实验设计概况：将4份生物学重复的第7天类胚体Stella阳性细胞，与2份生物学重复的第7天类胚体Stella阴性细胞进行比对分析。此外，还包含3份生物学重复的经荧光激活细胞分选得到的Stella阴性胚胎干细胞、2份生物学重复的经荧光激活细胞分选得到的Stella阳性胚胎干细胞，以及2份生物学重复的未分选StellaGFP胚胎干细胞。