Source Data for Supporting Information

Immunohistochemistry (IHC) was visualized by using an automatic multispectral imaging system with associated software (Vectra II, PerkinElmer, version 2.0.7.1). The size and zeta potential of HLPC and M@HLPC were measured by using a nanoparticle tracking analysis (NTA, Particle Metrix, Germany, version 8.05.04). The absorbance was measured by automatic microplate reader with associated software (Tecan Infinite M200, version 1.6.19.2). The interaction between each sub-component in HLPC were measured by microscale thermophoresis (MST, NT.115, Nanotemper, Germany). Flow cytometry data was acquired with Backman CytoFLEX LX Flow Cytometer with associated software (version 2.3.1.22). The biodistribution of nanoparticles were observed by using IVIS imaging system (PerkinElmer, USA, version 4.5.5).Power analysis of the animal experiments indicated that the chosen sample sizes per group are sufficient. We also referred to relevant literature to determine sample sizes. For the in vitro and in vivo experiments, we followed standards of good scientific practice. We used at least 3 biological replicates or 3 animals per group, to calculate means and standard deviations and to perform statistical analyses.Statistical analyses were performed using GraphPad Prism 8.0. Data were analyzed by two-tailed unpaired Student’s t-tests (two groups) or One-way ANOVA (multiple-groups). P values less than 0.05 were considered statistically significant.

免疫组织化学（Immunohistochemistry, IHC）的可视化成像采用搭载配套软件的自动多光谱成像系统完成，设备型号为Vectra II，生产商为珀金埃尔默（PerkinElmer），软件版本为2.0.7.1。HLPC与M@HLPC的粒径及Zeta电位通过纳米颗粒追踪分析（Nanoparticle tracking analysis, NTA）完成检测，检测设备由德国Particle Metrix公司制造，软件版本为8.05.04。吸光度通过搭载配套软件的全自动酶标仪完成检测，设备型号为Tecan Infinite M200，软件版本为1.6.19.2。HLPC各亚组分间的相互作用通过微尺度热泳（Microscale thermophoresis, MST）进行检测，设备型号为NT.115，生产商为德国Nanotemper公司。流式细胞术（Flow cytometry）数据通过贝克曼（Backman）CytoFLEX LX流式细胞仪及其配套软件采集，软件版本为2.3.1.22。纳米颗粒的体内生物分布通过IVIS成像系统进行观测，该系统由美国珀金埃尔默（PerkinElmer）公司生产，软件版本为4.5.5。动物实验的功效分析结果显示，每组选取的样本量均满足实验需求；本研究同时参考相关文献确定样本量。对于体外及体内实验，本研究严格遵循良好科学实践规范开展；每组实验至少设置3次生物学重复或3只实验动物，用于计算均值与标准差，并开展统计学分析。统计学分析采用GraphPad Prism 8.0软件完成；两组数据比较采用双侧非配对Student t检验，多组数据比较采用单因素方差分析（One-way ANOVA）；以P值小于0.05作为差异具有统计学意义的判定标准。