Macromolecular crystallography at the Philipps University Marburg (MarBAG)

Located at the Philipps-University-Marburg and the Max-Planck-Institute for terrestrial Microbiology in Marburg, Germany, the MarBAG employs X-ray crystallography to answer fundamental questions involving synthesis and assembly of macromolecular machines and cellular structures (e.g. CO2-fixation pathways, flagellar biosynthesis, CRISPR-Cas Systems, cell-wall-related processes). Our analysis of the structural basis of CO2 fixation and the synthesis of the green-house gas methane will help to engineer these processes as tools in climate control. Furthermore, we are interested in plant-pathogen interactions and the function of nucleotide derived signalling molecules in cellular stress physiology and organization. Currently, a 200 keV cryo-electron microscope is being installed at the UMR to complement our efforts to structurally characterize macromolecular complexes, which do not readily crystallize.

该数据集依托位于德国马尔堡的菲利普斯-马尔堡大学（Philipps-University-Marburg）与马克斯·普朗克陆地微生物研究所（Max-Planck-Institute for terrestrial Microbiology）开展研究，MarBAG团队采用X射线晶体学（X-ray crystallography）来解答涉及大分子机器与细胞结构的合成、组装的基础科学问题，例如二氧化碳固定途径（CO2-fixation pathways）、鞭毛生物合成（flagellar biosynthesis）、CRISPR-Cas系统（CRISPR-Cas Systems）及细胞壁相关过程等。我们对二氧化碳固定的结构基础与温室气体甲烷合成的分析，将有助于将这些过程改造为气候调控工具。此外，我们还关注植物-病原体互作，以及核苷酸衍生信号分子在细胞应激生理与细胞组织中的功能。目前，一台200 keV冷冻电子显微镜（cryo-electron microscope）正在UMR处安装，以辅助我们对不易结晶的大分子复合物进行结构表征。