Drosophila WDFY3/Bchs overexpression impairs neural function

Tab S1. Differentially expressed genes of glial Bchs overexpression flies. Gene expression levels from glial Bchs overexpression flies (repo>bchs) was compared to the respective controls (repo-Gal4/+ and UAS-bchs/+). Genes were considered to be significantly differentially expressed if adjusted p-value < 0.05. baseMean: mean of normalized counts of all samples; log2FoldChange: log2 difference of the gene expression level in Bchs overexpression flies compared to the control group; lfcSE: standard error of log2 fold change; stat: Wald statistic; pvalue: Wald test p-value; padj: p-value adjusted by Benjamini-Hochberg method.

Tab S2. Differentially expressed genes of neuronal Bchs overexpression flies. Gene expression levels from neuronal Bchs overexpression flies (nSyb>bchs) was compared to the respective controls (nSyb-Gal4/+ and UAS-bchs/+). Genes were considered to be significantly differentially expressed if adjusted p-value < 0.05. baseMean: mean of normalized counts of all samples; log2FoldChange: log2 difference of the gene expression level in Bchs overexpression flies compared to the control group; lfcSE: standard error of log2 fold change; stat: Wald statistic; pvalue: Wald test p-value; padj: p-value adjusted by Benjamini-Hochberg method.

Tab S3. Genes differentially expressed in glial and neuronal Bchs overexpression flies. Genes are listed which were differentially expressed in both, glial and neuronal Bchs overexpression flies. Glial/neuronal direction: states whether the gene expression was down- or up-regulated in glial and neuronal Bchs overexpression flies, respectively; concordance: states whether the gene expression was dysregulated in the same direction in glial and neuronal Bchs overexpression flies.

Tab S4. GO enrichment analysis from differentially expressed genes of glial Bchs overexpression flies. Gene differentially expressed in glial Bchs overexpression flies (repo>bchs) were subjected to GO enrichment analysis. GO modules were considered to be significantly enriched with a p-value after family wise error rate multiple correction (padj) < 0.05.

Tab S5. GO enrichment analysis from differentially expressed genes of neuronal Bchs overexpression flies. Gene differentially expressed in neuronal Bchs overexpression flies (repo>bchs) were subjected to GO enrichment analysis. GO modules were considered to be significantly enriched with a p-value after family wise error rate multiple correction (padj) < 0.05.

Tab S6. GO enrichment analysis from genes differentially expressed in glial and neuronal Bchs overexpression flies. Gene differentially expressed in both, glial and neuronal Bchs overexpression flies (repo>bchs and nSyb>bchs), were subjected to GO enrichment analysis. GO modules were considered to be significantly enriched with a p-value after family wise error rate multiple correction (padj) < 0.05.

Tab S1. 胶质细胞Bchs过表达果蝇的差异表达基因（differentially expressed genes）。将胶质细胞特异性过表达Bchs的果蝇（repo>bchs）的基因表达水平与对应的对照组（repo-Gal4/+及UAS-bchs/+）进行比较。以校正后p值（adjusted p-value）<0.05作为基因存在显著差异表达的判定标准。其中，baseMean为所有样本的标准化计数均值；log2FoldChange为Bchs过表达果蝇与对照组的基因表达水平的对数2倍差值；lfcSE为对数2倍变化的标准误；stat为Wald统计量（Wald statistic）；pvalue为Wald检验的原始p值；padj为经本杰米尼-霍赫贝格方法（Benjamini-Hochberg）校正后的p值。

Tab S2. 神经元Bchs过表达果蝇的差异表达基因。将神经元特异性过表达Bchs的果蝇（nSyb>bchs）的基因表达水平与对应的对照组（nSyb-Gal4/+及UAS-bchs/+）进行比较。以校正后p值<0.05作为基因存在显著差异表达的判定标准，各统计量含义与Tab S1一致。

Tab S3. 胶质细胞与神经元Bchs过表达果蝇的共有差异表达基因。本表格列出了同时在胶质细胞及神经元Bchs过表达果蝇中出现差异表达的基因。其中，Glial/neuronal direction字段用于说明该基因在胶质细胞与神经元Bchs过表达果蝇中分别为上调或下调表达；concordance字段用于说明该基因在两类细胞的Bchs过表达果蝇中是否呈现一致的表达失调方向。

Tab S4. 胶质细胞Bchs过表达果蝇差异表达基因的GO富集分析（Gene Ontology enrichment analysis）。对胶质细胞特异性过表达Bchs的果蝇（repo>bchs）中的差异表达基因开展GO富集分析。以经家族错误率多重校正后的p值（padj）<0.05作为GO模块显著富集的判定标准。

Tab S5. 神经元Bchs过表达果蝇差异表达基因的GO富集分析。对神经元特异性过表达Bchs的果蝇（repo>bchs）中的差异表达基因开展GO富集分析。以经家族错误率多重校正后的p值<0.05作为GO模块显著富集的判定标准。

Tab S6. 胶质细胞与神经元Bchs过表达果蝇共有差异表达基因的GO富集分析。对同时在胶质细胞及神经元Bchs过表达果蝇（repo>bchs及nSyb>bchs）中出现差异表达的基因开展GO富集分析。以经家族错误率多重校正后的p值<0.05作为GO模块显著富集的判定标准。