Expression modes and tagging systems for AP/MS screening.

The Arifuzzaman dataset is an outlier when compared with other AP/MS datasets. A possible explanation is that it is the only screen that combined both non-physiological protein expression and His-tagging instead of the superior tandem purification procedure. Note: by default AP/MS datasets are interpreted using the spoke model. In addition we list the Gavin network derived by socio-affinity scoring (scores above ). The Collins dataset relies on the same experimental data as the Krogan and Gavin datasets and is derived by a method similar to socio-affinity [7].

相较于其他亲和纯化质谱（Affinity Purification-Mass Spectrometry，AP/MS）数据集，Arifuzzaman数据集属于异类数据集。一种可能的解释为，该数据集是唯一同时采用非生理性蛋白表达与组氨酸标签（His-tag）标记，而非更优的串联纯化流程的筛选实验数据集。注：默认情况下，AP/MS数据集均采用辐条模型（spoke model）进行结果解读。此外，我们列出了经社会亲和性打分（socio-affinity scoring）得到的Gavin互作网络（打分阈值高于[原文未明确具体数值]）。Collins数据集与Krogan、Gavin数据集采用了相同的实验数据，其推导方法与社会亲和性打分法[7]相似。