Pattern of Extramammary Paget disease gene expression. Pattern of Extramammary Paget disease gene expression

To further understand different gene expression of Extramammary Paget disease skin and normal skin, we have employed whole skin microarray expression profiling as a discovery platform to identify different genes with Extramammary Paget disease skin and normal skin.comparision with normal skin, upgene is 4572 in disease group, downgene is 6473. mTOR pathway, RAS pathway, PI3K-AKT pathway, IL6-JAK-Stat3 pathway and so on are activated, WNT Pathway is inhibited. Overall design: 3 normal human skin and 4 Extramammary paget disease scrotum skin were taken and Quickly put it into liquid nitrogen ,the samples were taken to lab with dry ice,RNA were extracted with trizol,Good quality RNA were explored as microarrary analysis in capitalBio technology.

为深入解析乳腺外佩吉特病（Extramammary Paget disease, EMPD）皮肤与正常皮肤的基因表达差异，本研究采用全皮肤组织微阵列（microarray）表达谱作为筛选平台，以鉴定二者间的差异表达基因。与正常皮肤相比，病变组中共存在4572个上调基因、6473个下调基因。其中，mTOR通路、RAS通路、PI3K-AKT通路、IL6-JAK-STAT3通路等多条信号通路被激活，而WNT通路则受到抑制。实验设计详情如下：共采集3例正常人皮肤组织与4例乳腺外佩吉特病阴囊皮肤组织，样本获取后立即置入液氮中速冻，并通过干冰运输至实验室；采用Trizol试剂提取总RNA，经质检合格的高质量RNA样本将送往capitalBio生物技术公司进行微阵列分析。