RNA-seq Analysis of Kidney versus Splenic CD4+ and CD8+ T cells in MRL/lpr Lupus Mice

Purpose: To understand the differences in gene expression of lupus T cells in secondary lymphoic organ versus site of organ damage Overall design: Methods: CD4+ and CD8+ T cells from spleen and kidney of 14-week-old MRL/lpr mice were sorted, and their RNA extracted for deep sequencing, in duplicate. Sequenced reads were aligned to MM9 mouse genome using Star and Bowtie.

研究目的：探究狼疮T细胞在次级淋巴器官与器官损伤部位的基因表达差异。实验设计与方法：选取14周龄的MRL/lpr小鼠，对其脾脏与肾脏中的CD4+及CD8+ T细胞进行分选，提取RNA后开展双重复样深度测序；将测序得到的读段与MM9小鼠参考基因组进行比对，所用比对工具为Star与Bowtie。