Composition of the Sphagnum moss microbiome and its response to climate change drivers

The major objective of this study was to identify major microbial taxa associated with living Sphagnum moss, and elucidate microbial groups that are impacted by climate change drivers such as warming and elevated CO2. Microbial taxa were identified by 16S rRNA gene amplicon sequencing of DNA isolated from Sphagnum moss individuals. Sphagnum was collected from the Spruce and Peatland Responses Under Changing Environments (SPRUCE) experiment in the S1 bog present within the Marcell Experimental Forest in northern Minnesota.

本研究的核心目标为鉴定与存活泥炭藓（Sphagnum）相关的主要微生物类群，并阐明受气候变暖、CO₂升高等气候变化驱动因子影响的微生物类群。研究通过提取泥炭藓个体中的DNA，采用16S rRNA基因扩增子测序技术对微生物类群进行鉴定。泥炭藓样本采集自美国明尼苏达州北部马塞尔实验森林内S1泥炭沼泽中的「环境变化下云杉与泥炭地响应」（Spruce and Peatland Responses Under Changing Environments, SPRUCE）实验平台。