Allele-specific expression analysis of Masculinizer (Masc) gene knocked-down embryos in the silkworm, Bombyx mori. Bombyx mori strain:F1 hybrid (N4 x p50T)

The goal of this study is to know whether one of the two Z chromosomes is inactivated or both Z chromosomes are suppressed in Bombyx mori males. Transcriptome analysis was performed using Masc knocked-down hybrid embryos between two B. mori strains. The obtained polymorphic information can be used for allele-specific expression analysis.

本研究旨在探究家蚕（Bombyx mori）雄性个体中，两条Z染色体（Z chromosome）究竟是其中一条发生失活，还是两条均被抑制。本研究利用两个家蚕品系间的Masc基因敲低杂交胚胎开展转录组分析，所获多态性信息可用于等位基因特异性表达分析。