Effect of IL-12+IL-18 or anti-NK1.1 stimulation on splenic NK cells [mNK_Activation_RNA-seq]. Effect of IL-12+IL-18 or anti-NK1.1 stimulation on splenic NK cells [mNK_Activation_RNA-seq]

We stimulated freshly enriched splenic NK cells with either IL-12+IL-18 (1ng/ml each) or plate-bound anti-NK1.1. NKs from several C57Bl/6 mice were pooled. We then performed gene expression profiling analysis using data obtained from RNA-seq at two time points: 3 or 6 hours. Overall design: Comparative gene expression profiling analysis of RNA-seq data for unstimulated versus IL-12+IL-18 or anti-NK1.1 stimulated NK cells

我们使用终浓度均为1ng/ml的白细胞介素12（IL-12）联合白细胞介素18（IL-18），或固相包被的抗NK1.1抗体，对新鲜富集的脾脏来源自然杀伤细胞（Natural Killer cell, NK）进行刺激。收集多只C57BL/6小鼠的NK细胞并混合后，在3小时与6小时两个时间点通过RNA测序（RNA-seq）获取的数据开展基因表达谱分析。实验整体设计：对未刺激组、IL-12+IL-18刺激组及抗NK1.1抗体刺激组的NK细胞的RNA测序数据进行比较基因表达谱分析。