Isolation and genome sequencing of Streptomyces sp. from the soil in Sudan

This project is the continuation of the study that was described by Abdelrahman et al., 2022, in which 175 Actinomycetes strains were isolated from rhizosphere and bulk soil samples collected in 18 locations in Sudan. The strains' ability to inhibit the oomycete Phytophthora infestans was investigated. Considering the importance of elucidating the compounds underlying the observed anti-P. infestans activity, we sequenced the whole genome of several closely and distantly related Streptomyces strains that represent active and inactive groups against P. infestans to identify the strains to the species level as well as to use a comparative genomics approach which might give insights into the active metabolites that caused/induced the pathogen inhibition.

本研究是Abdelrahman等人2022年发表研究的延续。此前该研究从苏丹18个采样点采集的根际土壤与非根际土壤（bulk soil）样本中，分离得到175株放线菌（Actinomycetes）菌株，并对这些菌株抑制疫霉属病原菌致病疫霉（Phytophthora infestans）的能力进行了考察。鉴于阐明介导上述抗致病疫霉活性的活性化合物具有重要研究意义，我们对多株亲缘关系远近各异的链霉菌（Streptomyces）菌株开展了全基因组测序——这些菌株分别隶属于对致病疫霉具有抑制活性与无抑制活性的类群——以期通过该工作实现两个目标：一是将菌株精准鉴定至物种水平，二是采用比较基因组学（comparative genomics）方法，为揭示引发或诱导病原菌抑制的活性代谢物提供研究见解。