m6A/m-Seq of human B-lymphocyte cell lines from healthy controls and major depressive disorder patients

N6-Methyladenosine (m6A) and N6,2'-O-dimethyladenosine (m6Am) are abundant mRNA modifications that regulate transcript processing and translation. The role of both, here termed m6A/m, in the human stress response are currently unknown. Here, we provide m6A/m-Seq of immortalied cell lines derived from B lymphocytes from male healthy donors or male donors diagnosed with major depressive disorder (MDD), harvested 1 h after treatment with 100 nM cortisol or mock treatment. PolyA-RNA-fragments of each sample was processed both as m6A/m-sample (RNA immunoprecipiation RIP with an m6A and m6Am antibody) and RNA-input sample. Overall design: 3 biological replicates of cells from healthy male donors and 3 biological replicates of cells from male donors diagnosed with major depressive disorder each split in two treatment samples: mock treatment (ethanol only) or 100 nM cortisol in ethanol for 1 h creating 4 groups with each 3 biological replicates: healthy-mock: H_x_M, healthy-cortisol: H_x_C, MDD-mock: M_x_M and MDD-cortisol: M_x_C. Each samples was processed as m6A/m-immunoprecipitation-sample ("m6A") or as input RNA samples ("RNA"). Additionally, 1 RNA sample (equimolar mixed from all samples) was processed as IgG control.

N6-甲基腺苷（N6-Methyladenosine, m6A）与N6,2'-O-二甲基腺苷（N6,2'-O-dimethyladenosine, m6Am）是一类丰富的mRNA修饰，可调控转录本的加工与翻译过程。目前，这两种统称为m6A/m的修饰在人类应激反应中的作用尚不明确。本研究提供了源自健康男性供体，或确诊为重度抑郁症（major depressive disorder, MDD）的男性供体B淋巴细胞的永生化细胞系的m6A/m测序数据。所有细胞经100 nM皮质醇处理，或仅用乙醇进行模拟处理1小时后收获样本。每个样本的多聚腺苷酸化RNA（polyA-RNA）片段均被分为两组分别处理：一组为m6A/m样本（使用针对m6A与m6Am的抗体进行RNA免疫沉淀（RNA immunoprecipitation, RIP）），另一组为RNA输入对照样本。实验总体设计如下：健康男性供体细胞的3个生物学重复样本，与确诊为重度抑郁症的男性供体细胞的3个生物学重复样本，各自均被分为两个处理亚组：仅用乙醇进行的模拟处理，或用溶解于乙醇的100 nM皮质醇处理1小时，最终形成4组样本，每组各含3个生物学重复：健康-模拟组（"H_x_M"）、健康-皮质醇组（"H_x_C"）、MDD-模拟组（"M_x_M"）以及MDD-皮质醇组（"M_x_C"）。每一个样本均分别以m6A/m免疫沉淀样本（"m6A"）或输入RNA样本（"RNA"）的方式进行处理。此外，另有1份由所有样本等摩尔混合制备的RNA样本，作为IgG对照进行处理。