Range-wide whole genome data for three rough periwinkle species.. Littorina saxatilis

This dataset contains 482 short read samples from many sites covering the species' ranges of the rough periwinkles; Littorina saxatilis, Littorina arcana, and Littorina compressa. Littorina saxatilis samples are further subdivided into four ecotypes (Wave, Crab, Brackish and Barnacle) and an additional category for unassigned snails. Eight samples were collected from each site, species and ecotype, with a roughly even split of males and females. All samples were whole genome shotgun sequenced to a target of 10X coverage. Sequencing was split among two sequencing centres (137 samples with GeneWiz and 345 with Liverpool Centre for Genomic Research). Each samples at Liverpool was sequenced in three separate runs because of library preparation errors. A few samples had to be replaced in subsequent runs.

本数据集共包含482份短读长测序样本，采集自覆盖粗糙滨螺（rough periwinkles）三个物种——Littorina saxatilis、Littorina arcana与Littorina compressa——自然分布范围的多个采样点。其中Littorina saxatilis样本进一步划分为4类生态型：浪击型（Wave）、蟹捕食型（Crab）、咸淡水型（Brackish）与藤壶型（Barnacle），另有一类未分类滨螺样本。每个采样点、物种及生态型均采集8份样本，雌雄个体占比大致均衡。所有样本均采用全基因组鸟枪测序技术，目标测序覆盖度为10X。测序工作由两家测序中心承接：137份样本由GeneWiz完成测序，剩余345份样本由利物浦基因组研究中心（Liverpool Centre for Genomic Research）完成。因文库制备出现失误，利物浦中心的每份样本均开展了3次独立测序运行；部分样本在后续测序轮次中被替换。