Analysis of pvalb-egfp cells from mouse visual cortex.

We provide an annotated cDNA clone collection which is particularly suitable for transcriptomic analysis in the mouse brain. Using it on microarrays, we compared the transcriptome of EGFP positive and negative cells in a parvalbumin-egfp transgenic background and showed that more than 30 % of clones are differentially expressed. Our clone collection will be a useful resource for the study of the transcriptome of single cell types. Keywords: Cell type comparison Comparison between fluorescent and non-fluorescent cells isolated from the visual cortex of parvalbumin-egfp transgenic mice. Using five biological replicates with a dye-swap strategy (10 hybridisations).

本研究提供了一套经注释的互补脱氧核糖核酸(cDNA)克隆集，其尤其适用于小鼠脑内的转录组学分析。我们将该克隆集应用于微阵列实验，在小白蛋白-EGFP转基因背景下，对比了增强绿色荧光蛋白(EGFP)阳性与阴性细胞的转录组，结果显示超过30%的克隆存在差异表达。本克隆集将成为研究单一细胞类型转录组的宝贵资源。关键词：细胞类型对比；从小白蛋白-EGFP转基因小鼠视觉皮层分离的荧光与非荧光细胞间的对比；采用5次生物学重复结合染料置换策略（共10次杂交实验）