Translational profiling in undifferentiated spermatogonia

Germline-specific RiboTag IP-seq from postnatal testes synchronized for undifferentiated spermatogonia. Germline ribosomes were specifically tagged via Ddx4Cre, which activated expression of the RiboTag allele (Rpl22HA). Spermatogenesis in postnatal testes was chemically synchronized to obtain testes enriched for undifferentiated spermatogonia. For each synchronized sample collected from a single animal, RiboTag IP-seq and input RNA-seq was carried out. Synchronized samples lacking the RiboTag allele were used as negative controls for the IP-seq. RiboTag and control samples were each generated in triplicate.

本数据集源自经同步化处理以富集未分化精原细胞的出生后睾丸的生殖细胞特异性RiboTag免疫沉淀测序（RiboTag IP-seq）。研究通过Ddx4Cre特异性标记生殖细胞核糖体，该重组酶可激活RiboTag等位基因（Rpl22HA）的表达。为获取富集未分化精原细胞的出生后睾丸样本，研究通过化学手段同步化了出生后睾丸内的精子发生过程。针对每一份从单只实验动物中采集的同步化样本，均开展了RiboTag IP-seq与输入RNA测序（input RNA-seq）实验。未携带RiboTag等位基因的同步化样本被用作本次IP-seq实验的阴性对照。RiboTag实验组与对照组样本均设置了三次生物学重复。