Remodeling of active endothelial enhancers is associated with aberrant gene-regulatory networks in pulmonary arterial hypertension [RNA-seq]. Remodeling of active endothelial enhancers is associated with aberrant gene-regulatory networks in pulmonary arterial hypertension [RNA-seq]

Pulmonary Arterial Hypertension (PAH) is a cardiovascular disease characterized by progressively increasing blood pressure as a result of obliteration and loss of pulmonary arteries. We have extracted pulmonary arterial endothelial cells from lungs of a cohort of PAH patients (n=10) and controls (n=9), cultured the cells for 3-5 passages, and performed chromatin (H3K27ac, H3K4me1, and H3K4me3 ChIP-Seq), expression (RNA-Seq) and chromatin interaction profiling (ChIA-PET). We observed a large-scale remodelling of the active chromatin landscape at enhancers while promoters and gene expression remained unchanged. Overall design: Transcriptome profiling of pulmonary arterial endothelial cells of PAH-patients and donors.

肺动脉高压（Pulmonary Arterial Hypertension, PAH）是一类以肺动脉闭塞与缺失为病因、伴随血压进行性升高的心血管疾病。我们从PAH患者队列（n=10）与健康对照队列（n=9）的肺部组织中提取肺动脉内皮细胞，将细胞传代培养3-5代后，开展了多组学检测：针对组蛋白修饰H3K27ac、H3K4me1与H3K4me3的染色质免疫共沉淀测序（ChIP-Seq）、转录组测序（RNA-Seq）以及染色质互作谱分析（ChIA-PET）。研究结果显示，增强子区域的活性染色质景观发生了大规模重塑，而启动子区域与基因表达水平未出现显著改变。整体实验设计：对PAH患者与健康供体的肺动脉内皮细胞开展转录组谱分析。