RNAseq profiling of neonatal mouse Merkel cells and basal keratinocytes. RNAseq profiling of neonatal mouse Merkel cells and basal keratinocytes

Merkel cells (MCs), specialized neuroendocrine touch sensors within the epidermis, play a crucial role in tactile sensation. To gain deeper insignts into their molecular characteristics, we employed RNA sequencing (RNA-seq) to explore the transcriptome of MCs in comparsion to basal keratinocytes in neonatal mouse skin. Overall design: GFP-expression Merkel cells (GFP-positive) and control basal keratinocytes (GFP-negative, SCA1 and CD49F double positve) were isolated from the epidermis of Sox2-GFP reporter mice through Fluorescence-activated cell sorting. RNA extraction was conducted using the RNeasy® Plus Micro Kit from Qiagen. Library preparation was accomlished using the TruSeq Stranded Total RNA Library Prep – RS-122-2201 kit from Illumina. Subsequently, sequencing was performed on the HiSeq2500 platform, employing paired-end reads with a length of 125 base pairs.

梅克尔细胞（Merkel cells，MCs）是表皮内特化的神经内分泌触觉感受器，在触觉感知过程中发挥关键作用。为深入解析其分子特征，我们采用RNA测序（RNA sequencing，RNA-seq）技术，对比分析新生小鼠皮肤中梅克尔细胞与基底角质形成细胞的转录组特征。实验设计概述：通过荧光激活细胞分选术（Fluorescence-activated cell sorting），我们从Sox2-GFP报告基因小鼠的表皮中分离得到GFP阳性的梅克尔细胞，以及作为对照的GFP阴性、SCA1与CD49F双阳性的基底角质形成细胞。RNA提取采用Qiagen公司的RNeasy® Plus Micro Kit试剂盒。文库构建使用Illumina公司的TruSeq Stranded Total RNA Library Prep – RS-122-2201试剂盒完成。后续测序在HiSeq2500测序平台上开展，采用读长为125碱基对的双端测序模式。