Neuropixels datasets supporting main results of "Motor cortical output for skilled forelimb movement is selectively distributed across intratelencephalic and corticopontine projection neuron classes"

These are the core electrophysiological datasets supporting the conclusions of the work in the manuscript titled: "Motor cortical output for skilled forelimb movement is selectively distributed across intratelencephalic and corticopontine projection neuron classes". Primary datasets are Neuropixels recordings with reconstructed anatomical depth per unit and optotagging of a subset of neurons either Tlx3+ intratelencephalic neurons ("IT_") or Sim1+ corticopontine PT neurons ("PT_"). Briefly, we used a Neuropixels probe to record from the entire depth of mouse motor cortex and underlying striatum during performance of a operant joystick task. Simultaneous joystick position data (x,y) and licking at a reward port are found within associated "BehavioralVariables" data files within the directory structure. The physiological traces for each unit are the output of spike times and various useful alignments following spike sorting using JRClust and in most cases confirmed via parallel sorting with KiloSort. An associated Matlab script is provided that is sufficient to recreate the plots used in the manuscript. See related materials in the collection: https://doi.org/10.25378/janelia.c.5795939

本数据集为支持下述研究论文结论的核心电生理数据集，论文标题为："熟练前肢运动的运动皮层输出选择性分布于脑内投射与皮质桥脑投射神经元类群"。核心数据集包含带单单位重建解剖深度的神经像素（Neuropixels）记录，以及对部分神经元的光遗传学标记（optotagging）：Tlx3阳性脑内投射神经元（记为"IT_"）或Sim1阳性皮质桥脑锥体束神经元（记为"PT_"）。简言之，本研究在小鼠执行操作性操纵杆任务期间，使用神经像素探针记录小鼠运动皮层全层及下方纹状体的神经活动。目录结构下的"BehavioralVariables"数据文件中，包含同步采集的操纵杆位置（x、y轴）数据与奖励端口舔舐行为数据。各单单位的生理信号为经JRClust完成尖峰排序后得到的尖峰时间与各类有效对齐结果，且多数结果已通过KiloSort并行排序验证。配套提供了可复现论文中所用图表的Matlab脚本。相关数据集详见：https://doi.org/10.25378/janelia.c.5795939