Supplemental Figure 7

Supplemental Figure 7. Protection by ultrasound depends on an intact spleen. The protective effects of ultrasound were removed by prior splenectomy A) Study Design. Mice underwent splenectomy one week prior to sham or pUS treatment (Day 0). Twenty-four hours following pUS or sham, mice were injected with Folic Acid (FA). B) Plasma creatinine and C) Plasma BUN at 48h following FA. D) Percent change in body weight (BW) 48 hours after FA (Day 0). E) Kidney weight (right and left) at the time of harvest normalized to body weight of each mouse (Day 0). Data are represented as mean ± SEM. Significance was determined using the unpaired Student’s t test.

补充图7 超声介导的保护作用依赖于完整脾脏。预先实施脾切除术可消除超声的保护效应。
A) 实验设计：小鼠于第0天（即干预前一周）接受脾切除术，随后进行假手术或脉冲超声（pulsed ultrasound, pUS）处理。在脉冲超声或假手术处理24小时后，向小鼠注射叶酸（Folic Acid, FA）。
B) 叶酸注射后48小时的血浆肌酐水平；C) 叶酸注射后48小时的血浆尿素氮（Blood Urea Nitrogen, BUN）水平。
D) 叶酸注射后48小时（第0天）的体质量（Body Weight, BW）变化百分比。
E) 处死取材时采集的左右侧肾脏质量，以每只小鼠第0天的体质量进行归一化处理。
所有数据以平均值±标准误（standard error of the mean, SEM）表示。显著性差异采用非配对双侧Student t检验进行分析。