LMO3 is a suppressor of the basal-like/squamous PDAC subtype and reduces disease aggressiveness of pancreatic cancer through glycerol 3-phosphate metabolism

Pancreatic cancer is a heterogenous disease and consists of distinct subtypes. Here, we searched for candidate suppressor genes of the basal-like/squamous subtype, a more aggressive molecular subtype of pancreatic ductal adenocarcinoma (PDAC). Through an integrated transcriptome analysis, we identified the LMO3, as being downregulated in the basal-like/squamous PDAC using a discovery and validation approach. LMO3 downregulation associated with decreased patient survival. The goal of this study was aimed to identify LMO3 induced molecular signatures in PDAC. RNA was isolated from PDAC cell lines (n = 8 Panc 10.05_Ctrl vs LMO3) using TRIzol method. The mRNA profiles of the above human PDAC cell lines were generated by deep sequencing using Illumina NextSeq.

胰腺癌是一种异质性疾病，包含多种不同亚型。本研究针对侵袭性更强的分子亚型——基底样/鳞状亚型胰腺导管腺癌（pancreatic ductal adenocarcinoma, PDAC），筛选其潜在抑癌基因。通过整合转录组分析，采用发现-验证策略，我们鉴定出LMO3在基底样/鳞状PDAC中呈下调表达。LMO3的下调表达与患者生存期缩短显著相关。本研究旨在鉴定PDAC中LMO3诱导的分子特征。本研究采用TRIzol法从PDAC细胞系（n=8，分为Panc 10.05_Ctrl组与LMO3组）中提取RNA。上述人源PDAC细胞系的mRNA表达谱通过Illumina NextSeq平台完成深度测序获取。