Developmental trajectory of pre-hematopoietic stem cell formation from endothelium (scRNA-seq data set)

To examine the cell and molecular transitions between endothelial (E), hemogenic endothelial (HE), and intra-arterial clusters (IAC) cells, and the heterogeneity of hematopoietic stem and progenitor cells (HSPCs) within the IACs, we profiled ~40,000 cells from the caudal arteries (dorsal aorta, umbilical, vitelline arteries) of embryonic day (E) 9.5 to 11.5 mouse embryos by single-cell RNA sequencing (scRNA-Seq). We identified a developmental bottleneck in endothelial to hematopoietic transition (EHT) and heterogeneity within the IAC population.

为探究内皮细胞（Endothelial，E）、生血内皮细胞（Hemogenic Endothelial，HE）与动脉内簇（Intra-arterial Clusters，IAC）之间的细胞及分子转变过程，以及动脉内簇内造血干细胞和祖细胞（Hematopoietic Stem and Progenitor Cells，HSPCs）的异质性，我们对胚胎发育第9.5至11.5天小鼠的尾动脉（包含背主动脉、脐动脉及卵黄动脉）中的约4万个细胞开展了单细胞RNA测序（Single-Cell RNA Sequencing，scRNA-Seq）分析。本研究鉴定出内皮向造血转化（Endothelial to Hematopoietic Transition，EHT）过程中的发育瓶颈，以及动脉内簇群体内部的异质性。