A spontaneous mouse mutation in C57BL/6 at 105 mb on Chromosome 9.

A spontaneous recessive mutation arose in a C57BL/6 mouse colony at The Jackson Laboratory. The mutation causes bilateral progressive cerebral ischemia and premature death. The locus was mapped by standard genetic recombination techniques to mouse Chromosome 9 between 104.684 and 105.457 Mb. That region was resequenced using an Illumina GAIIx with 36 bp single end reads following array capture of the genomic DNA with a custom array made by Nimblegen. No mutations were identified.

自发隐性突变起源于杰克逊实验室（The Jackson Laboratory）的C57BL/6小鼠种群。该突变可引发双侧进行性脑缺血，并导致个体过早死亡。研究人员通过标准遗传重组技术将该基因座定位至小鼠9号染色体104.684至105.457 Mb区间。采用尼姆基因（Nimblegen）定制阵列对该区域的基因组DNA进行阵列捕获后，使用Illumina GAIIx平台以36 bp单端测序读段完成重测序，未检测到任何突变。