Dataset: Label-free detection of methicillin resistance in Staphylococcus aureus using different Raman-spectroscopy approaches

This is the dataset accompanying the submission of the manuscript: Label-free detection of methicillin resistance in Staphylococcus aureus using different Raman-spectroscopy approaches in the journal Microbiology Spectrum.

The data description is the following:

Strains
16859MRSA= Strain AUSTR-07-16859 MRSA
16859MSSA= Strain AUSTR-07-16859 MSSA
CC8MRSA= Strain 08V15773
CC8MSSA= Strain MRSA2010-174
AUSTR05MRSA= Strain AUSTR-05-15441 MRSA
AUSTR05MSSA= Strain AUSTR-05-15441 MSSA
CC361MRSA= Strain UAE-Abu Dhabi-020
CC361MSSA= Strain UAE-Dubai-80-MS 1368.9/09

Datasets
UVRR: UV-Resonance Raman with 244 nm excitation on bulk samples, calibration standard Polystyrene, measurements were time series of 10 consecutive spectra, for each strain and batch 25 time series were collected from 3 different slides
532nm: Single cell analysis with 532nm excitation, calibration standard 4AAP, one spectrum per bacterial cell was collected
785nm: Bulk analysis of bacterial colonies using 785 nm excitation and a Raman fibre probe, calibration standard 4AAP, bulk analysis, individual spectra of colonies were collected

Data structure is in the metadata files.
Individual spectra are in the folders sorted by the date they were measured.

本数据集随附投稿至《Microbiology Spectrum》期刊的研究手稿《采用不同拉曼光谱技术实现金黄色葡萄球菌甲氧西林耐药性的无标记检测》。

数据说明如下：

菌株信息：
16859MRSA：菌株AUSTR-07-16859 MRSA（甲氧西林耐药金黄色葡萄球菌，Methicillin-resistant Staphylococcus aureus）
16859MSSA：菌株AUSTR-07-16859 MSSA（甲氧西林敏感金黄色葡萄球菌，Methicillin-sensitive Staphylococcus aureus）
CC8MRSA：菌株08V15773
CC8MSSA：菌株MRSA2010-174
AUSTR05MRSA：菌株AUSTR-05-15441 MRSA
AUSTR05MSSA：菌株AUSTR-05-15441 MSSA
CC361MRSA：菌株UAE-Abu Dhabi-020
CC361MSSA：菌株UAE-Dubai-80-MS 1368.9/09

数据集详情：
UVRR：采用244 nm激发的批量样本紫外共振拉曼光谱检测，校准标准品为聚苯乙烯（Polystyrene）；检测模式为连续采集10张光谱的时间序列，针对每株菌株与每批次样本，从3张不同载玻片上共采集25组时间序列数据集
532nm拉曼：采用532 nm激发的单细胞拉曼光谱分析，校准标准品为4AAP；每株细菌细胞采集单张光谱数据
785nm拉曼：采用785 nm激发拉曼光纤探针对细菌菌落进行批量分析，校准标准品为4AAP；批量分析时采集菌落的单张光谱数据

数据存储结构：
数据结构信息存储于元数据文件中；单张光谱数据按测量日期分文件夹存储。