Soil protist communities across desertification and ecological restoration gradients in the Brazilian semiarid

This project investigates how desertification and long-term ecological restoration shape soil protist communities in the Brazilian semiarid region. Soil samples were collected from native vegetation, degraded desertified soils, and areas restored for nearly two decades through terracing and green manuring in Gilbues, Piaui Brazil. Protist communities were characterized using high-throughput sequencing of the 18S rRNA gene (V4 region) on the Illumina MiSeq platform. The dataset aims to evaluate how land degradation and restoration influence protist diversity, community composition, trophic structure, and ecological interactions in dryland soils.

本研究探究巴西半干旱地区荒漠化与长期生态修复如何塑造土壤原生生物（protist）群落结构。研究于巴西皮奥伊州吉尔布伊斯市（Gilbues, Piaui）开展，采集了原生植被区、退化荒漠化土壤区，以及通过梯田建设与绿肥种植实施近二十年生态修复的区域的土壤样品。依托Illumina MiSeq平台对18S核糖体RNA基因（18S rRNA gene）的V4可变区进行高通量测序，以表征原生生物群落特征。本数据集旨在评估土地退化与生态修复对旱地土壤中原生生物多样性、群落组成、营养结构及生态互作过程的影响。