Transcriptomic profiling of human CD34+ derived mast cells [RNA-Seq]

Transcriptome of human mast cells is extensively reorganized upon FcεRI crosslinking Human mast cells were generated from peripheral blood-derived CD34+ cells with recombinant hIL-3, hIL-6 and hSCF. Cells were left untreated or sensitized overnight with myeloma IgE (0.5 μg/ml) and treated with anti-IgE (1 μg/ml) for 2h.

高亲和力IgE受体（FcεRI）交联可使人肥大细胞的转录组（Transcriptome）发生广泛重塑。本研究所用的人肥大细胞由外周血来源的CD34+细胞，在重组人IL-3（hIL-3）、重组人IL-6（hIL-6）及重组人干细胞因子（SCF, hSCF）的诱导下分化获得。将细胞分为两组：一组不予任何处理作为空白对照，另一组以骨髓瘤IgE（0.5 μg/ml）致敏过夜，随后使用抗IgE抗体（anti-IgE，1 μg/ml）刺激2小时。