lncRNA and mRNA-sequencing of WS1 cells after 5Gy of X-Ray irradiation

Purpose: The goal of this study was to compare the lncRNA and mRNA expression profile of human fibroblast WS1 cells between 0 Gy and 5 Gy X-ray irradiation. Methods: lncRNA and mRNA-sequencing of WS1 cells were generated using Illuminal Hiseq4000, RNA-seq reads were mapped to human genome hg19 by hisat2 software. Cuffdiff software was used to analysis the differentially expressed levels of LncRNA and mRNA FPKM. Results: We identified different expressed levels of 88780 lnRNA and 20311 mRNA transcripts in WS1 cells between 0 Gy and 5 Gy X-ay irradiation. Conclusion: Our study first revealed the detail of lncRNA and mRNA expression profiles of WS1 cells after 5Gy of X-Ray irradiation Overall design: lncRNA and mRNA-sequencing of WS1 cells were generated using Illuminal Hiseq4000, RNA-seq reads were mapped to human genome hg19 by hisat2 software. Cuffdiff software was used to analysis the differentially expressed levels of LncRNA and mRNA FPKM.研究目的：本研究旨在比较人类成纤维细胞WS1在0 Gy与5 Gy X射线照射后的长链非编码RNA（lncRNA）和信使RNA（mRNA）表达谱。方法：采用Illumina Hiseq4000平台构建WS1细胞的长链非编码RNA与信使RNA测序文库，通过HISAT2软件将RNA测序读数比对至人类基因组hg19，并利用Cuffdiff软件分析长链非编码RNA与信使RNA的每百万片段转录本数（FPKM）的差异表达水平。结果：本研究在WS1细胞中鉴定出88780个长链非编码RNA转录本与20311个信使RNA转录本在0 Gy与5 Gy X射线照射后的差异表达情况。结论：本研究首次揭示了WS1细胞经5 Gy X射线照射后的长链非编码RNA与信使RNA完整表达谱细节。整体实验设计：采用Illumina Hiseq4000平台构建WS1细胞的长链非编码RNA与信使RNA测序文库，通过HISAT2软件将RNA测序读数比对至人类基因组hg19，并利用Cuffdiff软件分析长链非编码RNA与信使RNA的每百万片段转录本数（FPKM）的差异表达水平。