Gene expression data from cultured cortical neurons.. Mus musculus

We used Affymetrix DNA arrays to investigate the extent to which nuclear HDAC4 accumulation affects neuronal gene expression. Cultured neurons were infected with lentiviruses expressing either wildtype HDAC4 or the constitutuvely nuclear 3SA mutant under the control of neuronal Synapsin promoter. Overall design: Neurons were infected at 5 days after plating (5DIV) and analyzed at 14 DIV.

本研究采用Affymetrix DNA芯片（Affymetrix DNA arrays）探究细胞核内组蛋白去乙酰化酶4（HDAC4）的积累对神经元基因表达的影响程度。将培养的神经元使用分别携带野生型HDAC4或组成型核定位3SA突变体的慢病毒进行感染，上述慢病毒均受神经元突触蛋白（Synapsin）启动子调控。实验整体设计如下：神经元于接种后第5天（即体外培养第5天，5DIV）完成感染，并于体外培养第14天（14DIV）进行分析检测。