Data from immunocompromised individuals vaccinated with SARS-CoV2 vaccines: antibody titers, B cell receptor repertoire sequencing, and B cell immunophenotyping

The dataset contains B cell receptor (BCR) sequencing data. Two types of data are included:

Antigen-specific BCR sequences obtained by sorting B cells (CD19+, CD20+, IgG+, IgM-, Spike+) followed by PCR and Sanger sequencing for SARS-CoV-2. Sequences are provided per individual and also as a combined dataset.

Bulk BCR repertoire sequencing for IgM and IgG, with one FASTQ file per individual and sample.

All sequences are provided as FASTQ or FASTA files. Users will need to realign and re-annotate VDJ segments using their preferred reference database and software.

More details on the experimental design and sorting strategy can be found in the published paper: DOI 10.1002/cti2.70077

No direct identifiers are included; sample IDs are pseudonymized. The size of the dataset is approximately 30 GB.

本数据集包含B细胞受体（B cell receptor, BCR）测序数据，共涵盖两类数据：
1. 针对新型冠状病毒（SARS-CoV-2）的抗原特异性BCR序列：通过分选CD19阳性、CD20阳性、IgG阳性、IgM阴性、刺突蛋白阳性的B细胞，随后进行PCR扩增与桑格测序获得。序列按个体提供，同时也提供合并后的整合数据集。
2. 针对IgM与IgG的批量BCR受体组库测序数据：每个个体与样本对应一个FASTQ格式文件。
所有序列均以FASTQ或FASTA格式文件提供，使用者需使用自选的参考数据库与软件，对序列进行重比对与VDJ基因片段的重新注释。
有关实验设计与细胞分选策略的更多细节，请参见已发表论文：DOI 10.1002/cti2.70077。
本数据集未包含直接识别信息，样本ID均经过伪匿名化处理，总大小约为30 GB。