Studies on lactic acid bacteria isolated from fructose rich niches

\tThe dataset consists of results of various experiments carried out for screening and isolation of fructophilic lactic acid bacteria capable of potential probiotic properties such as tolerance to acid and simulated gastric juice, resistance to bile salt, antagonistic properties, antibiogram, haemolytic activity, characterization of fructophilic lactic acid bacteria and its suitability for commercial applications. In the present investigation, 13 fructophilic lactic acid bacterial strains were isolated from the ecological niches such as flowers and seeds.Among these strains, a potent fructophilic strain Fructobacillus fructosus MCC 3996 was isolated and selected for further studies since it appeared to be the best potential probiotic candidate. This isolate was characterized based on biochemical and cultural characteristics. Its identification has been confirmed by using 16S rRNA sequencing. The nutritional characteristics and incubation conditions for maximum fructophilicity was optimized. SDS-PAGE study demonstrated the presence of S-layer proteins obtained from the F. fructosus MCC 3996. Besides this, the strain showed the high free radical scavenging activity of F. fructosus MCC 3996 using DPPH and ABTS assay. Viability among the storage at 4 suggests the suitability of strain as a promising probiotic candidate for application in the food industry. Furthermore, it exhibited higher degrees of in vitro cholesterol assimilation in broth and showed a significant anti-hyperlipidemic effect (in vivo) in Swiss albino mice model. The application of F. fructosus in probioticated apple juice was also studied which found greater survival (7.60 ± 0.11 log10 cfu/mL), acceptable colour, aroma, and turbidity was achieved during refrigerated storage at 4 , up to 28 days.

本数据集包含为筛选、分离具有潜在益生特性的嗜果糖乳酸菌（fructophilic lactic acid bacteria）所开展的多组实验结果，相关特性涵盖耐酸性与模拟胃液耐受性、耐胆盐性、拮抗特性、药敏谱、溶血活性，以及嗜果糖乳酸菌的鉴定分析与商业化应用潜力评估。本研究从花朵与种子等生态位中分离得到13株嗜果糖乳酸菌菌株。其中，一株性能优异的嗜果糖菌株Fructobacillus fructosus MCC 3996被筛选用于后续研究，因其被证实为最优的潜在益生候选菌株。该分离株通过生化与培养特性完成表征，并借助16S rRNA测序确认了其物种身份。研究优化了可实现最大嗜果糖性能的营养条件与培养条件。十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）分析证实，该菌株（F. fructosus MCC 3996）存在S层蛋白。此外，通过1,1-二苯基-2-三硝基苯肼（DPPH）与2,2'-联氮-双-3-乙基苯并噻唑啉-6-磺酸（ABTS）试验测定显示，该菌株具备较高的自由基清除活性。4℃储存条件下的菌株存活率留存情况表明，该菌株可作为极具应用前景的益生候选菌株用于食品工业。进一步研究发现，该菌株在肉汤培养基中展现出较高的体外胆固醇同化能力，并在瑞士白化小鼠模型中表现出显著的抗高脂血症效应。本研究还探究了Fructobacillus fructosus在益生菌化苹果汁中的应用效果，结果显示，在4℃冷藏储存长达28天的过程中，该菌株仍可保持较高的存活率（7.60 ± 0.11 log₁₀ CFU/mL），且产品的色泽、香气与浊度均达到可接受标准。